recombinant protein eno1  (MedChemExpress)

Journal: Frontiers in Oncology

Article Title: ENO1 contributes to 5-fluorouracil resistance in colorectal cancer cells via EMT pathway

doi: 10.3389/fonc.2022.1013035

Figure Lengend Snippet: Expression of ENO1 in CRC tissues. (A) Representative immunohistochemical (IHC) images showing in situ ENO1 expression in colorectal cancer (CRC) and normal tissues (scale bar = 100 μm). (B) IHC scores of ENO1 in CRC vs normal tissues. (C) IHC scores of ENO1 in T I-II vs T III-IV tissues. (D–F) Overall survival of CRC patients with high and low expressions (D) of ENO1 with TNM staging I-II (E) and III-IV (F) . Statistical analyses were performed via two-tailed Student t test. ****, P ≤ 0.001.

Article Snippet: The sections were incubated with 1:100 diluted polyclonal anti-human ENO1 (BOSTER, Wuhan, China) at 4°C overnight.

Techniques: Expressing, Immunohistochemical staining, In Situ, Two Tailed Test

Journal: Frontiers in Oncology

Article Title: ENO1 contributes to 5-fluorouracil resistance in colorectal cancer cells via EMT pathway

doi: 10.3389/fonc.2022.1013035

Figure Lengend Snippet: Relationship between ENO1 and clinic-pathological factors in CRC patients.

Article Snippet: The sections were incubated with 1:100 diluted polyclonal anti-human ENO1 (BOSTER, Wuhan, China) at 4°C overnight.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: ENO1 contributes to 5-fluorouracil resistance in colorectal cancer cells via EMT pathway

doi: 10.3389/fonc.2022.1013035

Figure Lengend Snippet: Results of univariate and multivariate analyses of postoperative patients’ survival by Cox’s proportional hazard model.

Article Snippet: The sections were incubated with 1:100 diluted polyclonal anti-human ENO1 (BOSTER, Wuhan, China) at 4°C overnight.

Techniques:

Journal: Frontiers in Oncology

Article Title: ENO1 contributes to 5-fluorouracil resistance in colorectal cancer cells via EMT pathway

doi: 10.3389/fonc.2022.1013035

Figure Lengend Snippet: Subgroup analysis for the influencing factor of survival among colorectal cancer patients according to ENO1 expression. Statistical analyses were performed via two-tailed Student t test. *, P ≤ 0.05.

Article Snippet: The sections were incubated with 1:100 diluted polyclonal anti-human ENO1 (BOSTER, Wuhan, China) at 4°C overnight.

Techniques: Expressing, Two Tailed Test

Journal: Frontiers in Oncology

Article Title: ENO1 contributes to 5-fluorouracil resistance in colorectal cancer cells via EMT pathway

doi: 10.3389/fonc.2022.1013035

Figure Lengend Snippet: ENO1 expression was increased in the constructed 5-fluorouracil (FU)-resistant cells. (A) Comparison of ENO1 expression between various CRC cell lines in Cancer Cell Line Encyclopedia (CCLE). (B, C) Immunoblotting results of ENO1 (B) in cell lines HCT116, SW620, HT29, SW480, and LOVO and quantitative counting (C) . (D) Cell viability comparison between HCT116 cells and 5-FU-resistant cells. (E, F) Increase in ENO1 expression (E) in 5-FU-resistant cells and the statistical graph (F) . Statistical analyses were performed via two-tailed Student t test. *,P ≤ 0.05; **,P ≤ 0.01.

Article Snippet: The sections were incubated with 1:100 diluted polyclonal anti-human ENO1 (BOSTER, Wuhan, China) at 4°C overnight.

Techniques: Expressing, Construct, Comparison, Western Blot, Two Tailed Test

Journal: Frontiers in Oncology

Article Title: ENO1 contributes to 5-fluorouracil resistance in colorectal cancer cells via EMT pathway

doi: 10.3389/fonc.2022.1013035

Figure Lengend Snippet: Knockdown of ENO1 inhibited the proliferation of 5-fluorouracil (FU)-resistant colorectal cancer (CRC) cells. (A, B) Immunoblots (A) showing ENO1 protein levels in NC and ENO1-KD group and the quantified pattern (B) , reflecting the success of transfection. (C, D) Cell viability of HCT/5-FU (C) and SW620/5-FU (D) cells in the ENO1-KD group and NC group. (E, F) Proliferation rates (E) and colony forming capacity (F) of 5-FU-resistant CRC cells in the ENO1-KD and NC groups (scale bar = 6mm). Statistical analyses were performed via two-tailed Student t test. *,P ≤ 0.05; **,P ≤ 0.01; ***,P ≤ 0.001.

Article Snippet: The sections were incubated with 1:100 diluted polyclonal anti-human ENO1 (BOSTER, Wuhan, China) at 4°C overnight.

Techniques: Knockdown, Western Blot, Transfection, Two Tailed Test

Journal: Frontiers in Oncology

Article Title: ENO1 contributes to 5-fluorouracil resistance in colorectal cancer cells via EMT pathway

doi: 10.3389/fonc.2022.1013035

Figure Lengend Snippet: ENO1 knockdown inhibited the migration of resistant cells and was associated with the EMT process. (A–C) Display of representative photographs (A) in the ENO1-KD and NC groups and the number of migration cells in HCT116/5-FU cells (scale bar = 100 μm) (B) and SW620/5-FU cells (C) . (D, F) Western blotting of E-Cadherin, N-Cadherin, and Vimentin expression levels following knockdown of ENO1 in HCT116/5-FU cells (D) and SW620/5-FU cells (F) . (E, G) The bands were quantified and are presented as mean ± SEM of three independent experiments in HCT116/5-FU cells (E) and SW620/5-FU cells (G) . Statistical analyses were performed via two-tailed Student t test. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.

Article Snippet: The sections were incubated with 1:100 diluted polyclonal anti-human ENO1 (BOSTER, Wuhan, China) at 4°C overnight.

Techniques: Knockdown, Migration, Western Blot, Expressing, Two Tailed Test

Journal: Frontiers in Oncology

Article Title: ENO1 contributes to 5-fluorouracil resistance in colorectal cancer cells via EMT pathway

doi: 10.3389/fonc.2022.1013035

Figure Lengend Snippet: The regulation of the EMT process by ENO1 is associated with energy metabolism. (A) The morphological changes during the EMT process were suppressed in the ENO1-KD group compared with those in the NC group (observed by light microscope at 200× magnification). (B, C) Western blotting of E-Cadherin, N-Cadherin, and Vimentin expression in NC and ENO1-KD groups treated with or without 20 mM 2-DG for 4 h in HCT116/5-FU cells (B) and SW620/5-FU cells (C) . (D-E) The bands were quantified and are presented as mean ± SEM of three independent experiments in HCT116/5-FU cells (D) and SW620/5-FU cells (E) . Statistical analyses were performed via two-tailed Student t test. **, P ≤ 0.01; ***, P ≤ 0.001. ns, no significance.

Article Snippet: The sections were incubated with 1:100 diluted polyclonal anti-human ENO1 (BOSTER, Wuhan, China) at 4°C overnight.

Techniques: Light Microscopy, Western Blot, Expressing, Two Tailed Test

Journal: Frontiers in Oncology

Article Title: ENO1 contributes to 5-fluorouracil resistance in colorectal cancer cells via EMT pathway

doi: 10.3389/fonc.2022.1013035

Figure Lengend Snippet: ENO1 knockdown suppresses tumor growth in colorectal cancer (CRC) in vivo . (A, B) Total body weight and tumor volume in ENO1-KD and NC groups. (C, D) Total body weight and tumor volume in ENO1-KD+5-FU and 5-FU groups. (E) Representative pictures of subcutaneous tumors harvested from the four groups. (F) The weights of tumor masses with or without treatment. (G) Stratification of mice into cluster 1 (grey) and cluster 2 (blue) according to tumor weight and ENO1 levels. (H) Percentage of NC and ENO1-KD mice in each cluster. Statistical analyses were performed via two-tailed Student t test. *, P ≤ 0.05; **, P ≤ 0.01.

Article Snippet: The sections were incubated with 1:100 diluted polyclonal anti-human ENO1 (BOSTER, Wuhan, China) at 4°C overnight.

Techniques: Knockdown, In Vivo, Two Tailed Test